Mutating miR-71 drastically reduces the survival rate of animals in L1 diapause, and the effect can be suppressed by mutations of insulin receptor pathway genes age-1 and unc-31. The effect observed in ain-1(lf) mutants is likely the consequence of the combined effects of attenuating functions of these individual miRNAs. To identify individual miRNAs that play prominent roles in L1 diapause, we screened 72 available mutant strains of individual miRNAs and miRNA families (87 miRNAs in total) using the L1 starvation assay. (B) Survival rate of single and double mutants to indicate the functional relationship between ain-1 and age-1.
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